Robust design and optimization of retroaldol enzymes
نویسندگان
چکیده
منابع مشابه
Robust design and optimization of retroaldol enzymes.
Enzyme catalysts of a retroaldol reaction have been generated by computational design using a motif that combines a lysine in a nonpolar environment with water-mediated stabilization of the carbinolamine hydroxyl and β-hydroxyl groups. Here, we show that the design process is robust and repeatable, with 33 new active designs constructed on 13 different protein scaffold backbones. The initial ac...
متن کاملRobust Design and Reliability-based Design Optimization
A large number of problems in manufacturing processes, production planning, finance and engineering design require an understanding of potential sources of variations and quantification of the effect of variations on product behavior and performance. Traditionally, in engineering problems uncertainties have been formulated only through coarse safety factors. Such methods often lead to overdesig...
متن کاملRobust Design Optimization
Within the robust design optimization, the statistical variability of the design parameter is considered. The most general method for solving optimization problems under uncertainties is the well established Monte Carlo simulation method. However, the major shortcoming of this approach is its vast need of computational resources (the number of solver runs required), and these cannot be presumed...
متن کاملRobust Coordinated Design of UPFC Damping Controller and PSS Using Chaotic Optimization Algorithm
A Chaotic Optimization Algorithm (COA) based approach for the robust coordinated design of the UPFC power oscillation damping controller and the conventional power system stabilizer has been investigated in this paper. Chaotic Optimization Algorithms, which have the features of easy implementation, short execution time and robust mechanisms of escaping from local optimum, is a promising tool fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2012
ISSN: 0961-8368
DOI: 10.1002/pro.2059